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Cytoskeleton Web alert
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| Cytoskeleton The Gard lab at the University of Utah |
The
Gard laboratory (University of Utah, USA) studies cytoskeletal organisation
during oogenesis and teh development of Xenopus. Press on 'Introduction
to the cytoskeleton in Xenopus' to find a discussion of microtubules, intermediate
filaments and microfilaments. Actin, keratin and microtubule organisation
during oogenesis is described, with a comprehensive series of images. The
roles of XMAP215 and XMAP230 in microtubule assembly and stabilisation are
covered, and some useful links to other sites are provided. |
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| QuickTime movies (and MPEG format) are available from the videomicroscopy page of the Dept of Medical Physiology (Texas A&M University System Health Science Center). Zawieja presents a movie of protein kinase C and actin distribution in a toad stomach smooth muscle cell, which was recorded using immunolabelling and a CCD camera. | ||
| Actin assembly during cell movement | This site presents a movie from TM Terry (Uni of Connecticut, Storrs) showing actin dynamics during cell movement. | |
| Actin dynamics during pathogen invasion | This page contains movies from J Theriot's laboratory homepage (Stanford University Center for Molecular and Genetic Medicine). Several movies illustrate actin comets formed either during Listeria monocytogenes and Shigella flexneri infection or associated with ActA-coated bead motility in Xenopus extract. | |
| Cortical actin patches in budding yeast | This QuickTime movie (MPEG format also available) is from T Doyle's website (Botstein laboratory). Using a GFP-tagged version of actin, you can follow the polarization of the cortical patches in diploid yeast and follow their movement in haploid budding yeast. | |
| Microtubules
Microtubule dynamics in S. Pombe |
Movies from the website of the Molecular Motor Group (Marie Curie Research Institute, Surrey, UK). When we visited the site the movies were essentially related to GFP-microtubule dynamics in Schizosaccharomyces Pombe. Also available on the site are protocols for actomyosin and microtubule motility assays. | |
| Salmon Lab Movies | This is a collection of great movies on microtubule dynamics during mitosis, cell motility and membrane traffic. | |
| Zytoskellett | This website from Eckhard Mandelkow provides pages with information and pictures on the microtubule-associated Tau protein, on microtubule affinity regulating kinases (MARK) and on the structure of monomeric and dimeric kinesin (from rat brain). | |
| Motors
Kinesin Superfamily Protein (KIF) Home Page |
This page was constructed by Yasuski Okada from Nobutaka Hirokawa's laboratory at Tokyo University. It offers a phylogenetic tree and lists of KIFs by organism, class and family. |
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| The Vale Lab Home Page | The homepage of the Vale laboratory offers pictures and movies from the Nature review article published by Rice et al. (S Rice et al., Nature 1999,402:778-783). As well as numerous movies of kinesin (GFP constructs moving on a microtubule track and a microtubule gliding on myosin coated on glass) and a movie of Katanin severing, this site also presents an animated model for muscle myosin-based motility. | |
| Kinesin movement along a microtubule | This site from the Gelles laboratory has a movie of a single kinesin motor moving along a microtubule. When the motor pauses this indicates the time that it is bound to AMP-PNP. The work was published in Biochemistry (Y Vugmeyster, E Berliner, Jeff Gelles, Biochemistry 1998, 37:7470757). | |
| Yoshio Fukui Homepage | This site has an historic picture of the colocalization of myosin II and actin filaments in Dictyostelium (the photo is dedicated to the late Philip Presley, a former Zeiss representative at the Marine Biological Laboratory, Woods Hole, Massachusetts). Several time-lapse movies of cytokinesis and locomotion in Dictyostelium are available here. The images in some of the sequences are pixilated but the content is very good. | |
| This site has movies and animations of vesicle transport on microtubules and actin filaments in axoplasm of the squid giant axon. The animation shows the tail-tail interaction of kinesin and myosin V on vesicles and the transition of vesicles from the microtubules to actin filaments. | ||
| Neurofilaments Neurofilament/microtubule movies | This page is from the Cell Biology and Cytoskeleton group at the Brigham and Women's hospital (Harvard Medical School). It provides basic information on neurofilaments and movies of neurofilament translocation along microtubules that are either polarity marked or not. | |
| Cell
motility Borisy Lab Movie Page |
This page has around 50 different movies illustrating the behaviour of the tubulin and actin cytoskeletons in cell movement, axonal growth and pigment organisation in melanophores. It is fascinating. | |
| Retinal dendrites | This site from Rachel Wong's lab shows a number of images and movies of retinal dendrites. | |
| Miscellaneous
WWW virtual library |
This is a rather complete list of links to the homepages of laboratories working on the cytoskeleton. | |
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Updated: 11:00 31/3/05
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